gene clone

Objective To clone human ornithine decarboxylase antizyme ( OAZ ) mutation gene and express its recombinant protein.

目的构建人鸟氨酸脱羧酶抗酶 ( OAZ ) 突变基因,重组至原核表达载体中并表达出重组蛋白.

Objective To clone the human recombined bone morphogenetic protein 2 ( BMP 2 ) gene in Escherichia coli.

目的:在大肠杆菌中 克隆人 骨形成蛋白2基因.

Transgenic Organism Selectable Maker Gene and Control Gene Clone and Detection Technology Research

转基因生物选择标记基因与对照基因及检测技术初步研究

Clone the full-length sequence of tat gene of a HIV-1 B strain from Henan Province of China, and compare the similarity of its encoding product with known tat protein.

克隆河南人免疫缺陷病毒（HIV）感染者HIV-1B型株tat基因完整编码框序列，并分析比较其编码产物的序列结构特点。

For the past few years, with the further of genetics research aimed to pathological myopia, the scholars utilize the techniques such as genome scan 、 candidate gene screening 、 linkage analysis to locate the related gene of pathological myopia and the candidate gene clone.

近年来，国内外学者对高度近视的遗传学研究进一步深入，已应用全基因组扫描和候选基因筛查、连锁分析等技术进行病理性近视相关基因定位和候选基因克隆定位工作。

Objective To clone, identify, and express the staphylococcal enterotoxins A ( SEA) gene from Staphylococcus aureus in order to prepare monoclonal antibody and diagnostic reagent.

目的对金黄色葡萄球菌肠毒素A基因（SEA）进行克隆、鉴定与表达，为制备单抗、诊断试剂及进一步深入研究SEA的功能奠定基础。

Gene clone and nucleotid sequence analysis of grapevine leafroll virus 3

葡萄卷叶伴随病毒基因的克隆及序列分析

Objective To clone and express Trp operon gene of E.

目的克隆并表达大肠杆菌色氨酸操纵子基因,提高其酶活性.

Objective: Our aim was to clone P 1 protein structure gene of mycoplasma pneumoniae in escherichia coli.

目的: 在大肠杆菌中克隆肺炎支原体P1蛋白结构基因,为实现肺炎支原体P1蛋白结构基因的大量扩增及表达奠定基础.

Gene clone and expression of the specific fragment of flagellin from Borrelia burgdorferi

伯氏疏螺旋体鞭毛蛋白特异性区段的基因克隆和表达

Aim To clone protein phosphatase 2C ( PP2C) gene from Leishmania donovani 1S parasite.

目的克隆杜氏利什曼原虫（Leishmania donovani，Ld）1S株蛋白磷酸酶2C（PP2C）的编码基因，为应用这种编码T细胞抗原的基因进行基因疫苗研究奠定基础。

A new gene clone technology was used to construct the expression vector.

利用一步法克隆新技术构建表达质粒，并转染到大肠杆菌中进行表达。

One clone that hemolytic activity indicating the presence of an intact gene was identified.

其中一株具溶血活性,显示该选殖株所含的重组质体带有完整的细胞溶解毒素基因.

The bulk DNA was isolated from bacterial habits in sediments of the Xisha trough, the south China sea. Using a pair of primers special for most common bacteria, the researchers amplified partial sequences of bacterial 16S rDNA gene and thus constructed a 16S rDNA clone library.

对来自南海西沙海槽的深海沉积物进行总DNA提取，并构建细菌16S rdna文库。

Gene Clone of Thick Filament Gene in B. Mandarina and B. Mori fibers from silkworm cocoons provide threads for knitting.

家蚕和野桑蚕粗肌丝结构基因的研究由蚕茧产生的纤维，提供针织品所需的细丝。

Positive selection and vaccine mechanism are introduced on the premise of negative selection and clone selection. And a strategy of DNA PRI in the detection is adopted by using gene detection.

该算法综合了生物免疫系统的先天性免疫和适应性免疫机制，在负选择和克隆选择的基础上又加入了阳性选择和疫苗机制，并利用基因检测实现了检测的DNA优先级策略。